The Innovation Centre offers a Sanger Sequencing Service using Applied Biosystem's 3730xl DNA Analyzer technology.
In addition to the regular service, the Innovation Centre also offers the 24hr BaseXpress Sequencing Service.
Sanger sequencing is often used to proofread plasmid DNA sequence to verify the integrity of a gene inserted into an expression vector (consult the Plasmid DNA extraction User Guide) or to re-sequence targeted regions of a genome with the purpose of finding genetic variations (see Sanger based SNP discovery service).
Sanger sequencing is most suitable for small to medium range sequencing projects. For large scale sequencing projects, please contact the Client Management Office to help select the most suitable Next-Generation sequencing technology.
More details can be found in the User Guide.
In addition to the Sanger Sequencing Service, the Innovation Centre offers a plasmid DNA extraction service based on the alkaline lysis method. This service is only available in 96-well format and must include the sequencing of the extracted plasmid DNA. Consult the User Guide to learn about submission requirements.
The Sanger sequencing methodology is described in the following web sites:
For a description of the 3730xl technology, Applied Biosystems website may be consulted.