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Sequenom® iPLEX® Gold Technology

Sequenom® iPLEX® Gold Genotyping Technology

  • Analyze between 5 and 400 loci (SNP and indel) in parallel
  • Custom genotyping for studying focused genomic regions of interest on any species

Applications

  • Candidate-gene studies
  • SNP discovery validation
  • Fine-mapping of candidate association regions
Description

The Sequenom® iPLEX® Gold genotyping technology is ideal for medium sized projects (for SNP validation or fine-mapping studies) when scoring between 5 and 400 bi-allelic markers such as SNPs and indels (insertion/deletion polymorphisms) on hundreds to thousands of DNA samples is needed. A major advantage with this technology is that it is highly flexible since there are no SNP type restrictions for the construction of the panel. It also allows the genotyping of varied types of polymorphisms in any organism. The panel can contain up to 36 markers.

The genotyping reaction is based on a multiplex PCR followed by a template-directed single base extension (SBE) using a probe. The products are then separated and detected by mass spectrometry (MALDI-TOF MS). The assay conversion rate is between 85% depending on the projects. The call rate is about 92% and the error rate is less than 0.1 %.

For more details : Ehrich et al., 2005 Nucl Acids Res 33:e38 or the Sequenom® website.

Available Products

The design of custom assays is realized solely by our personnel.

Information and Service Request

The Client Management Office can be contacted for more information and for service request.