Microarray-based Comparative Genomic Hybridization (aCGH) is a powerful technique for identification and characterization of copy number variation (CNV) across the genome (CNV analysis and cytogenetic studies). CGH microarrays provide high-resolution and flexibility for genome-wide or customized zoom-in profiling of genomic aberrations without amplification or complexity reduction. In order to ensure maximal sensitivity and specificity, the design and the selection of the probes have been rigorously optimized and validated.
The DNA copy number changes in the experimental sample are measured relative to a reference sample. The experimental and the reference samples are differentially labeled using a two-color process, then mixed together, and hybridized onto a microarray. The fluorescence signal is detected with a scanner, and the results are analyzed using a CNV analysis software.
Agilent microarrays are manufactured using SurePrint technology, a proprietary non-contact industrial inkjet printing process. Using an in situ synthesis process, 60-mer length oligonucleotide probes are deposited, base-by-base, uniformly onto specially-prepared glass slides, resulting in consistent spot uniformity and traceability. A real-time quality control inspection system ensures high quality microarrays.
Agilent's web site can be visited for more details.
Agilent DNA microarrays are designed for several high-resolution genomic applications (Oligo aCGH, Gene Expression, DNA Methylation, ChIP-on-chip & microRNA). For the moment, only aCGH application is offered. Gene Expression will be available soon.
The Client Management Office can be contacted for more information and for service request.